Do you have to use it with a nalgene-type container? Would it work with a flexible bag-like container?

Bag should work fine I think. As long as the mouth is wide enough to fit the whole pen in.

Will the water in my Hydroflask stay purified after using the Steripen if I’m not drinking the whole bottle at once, or do have to repeat the process?

my Daughter and I used it for a week on the Appalachian Trail and we were fine. We did not re-sterilize our water bottle every time we drank from it—only using the steri pen when we initially filled the water bottle. We also used it to sterilize the water we poured into our 3 liter Camelbacks. Note that there were a couple of contaminated water advisories posted when we went, we used two purification systems-the Sawyer mini and the Steripen-just to be safe, but we never re-purified each time we drank.

Just confirming that the charging comes with it?

Depends on what you mean by "the charging." The cord comes in the box (although it's a generic cellphone type cord, anyway). A wall charger does not. Any charger into which you can plug the cable will suffice, as the SteriPen does not require a high-output wall unit.

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💧 Purify your adventure—because safe water is the new luxury.

POWER UP ANYWHERE - USB rechargeable with any laptop, solar panel, or car adapter for ultimate on-the-go freedom.
INSTANT SAFE HYDRATION - Drink water immediately once the smiley lights up—no waiting, no guessing.
LIGHTWEIGHT PORTABLE - At just 340g and compact dimensions, it fits effortlessly in your backpack or briefcase.
SMART BATTERY INDICATOR - Stay ahead of your hydration game with a clear, user-friendly display showing battery status.
ADVANCED UV C STERILIZATION - Clinically proven to eliminate 99.9% of protozoa, bacteria, and viruses for peace of mind.

The SteripenUltra UV Water Purifier uses powerful UV-C light to eliminate 99.9% of harmful pathogens in clear water, delivering instant safe drinking water. Lightweight and USB rechargeable, it treats up to 50 liters per charge and features a smart battery display for hassle-free use during outdoor adventures or daily commutes.