What do you put inside these?

Anything you want. I use mine to put in Ammonium granules to filter out the ammonia. It fits behind my filter cartridge. I like to think that it has been effective.

I'll be using two of these, and two disposable .Does it matter which is in front and which is in back? Is this overkill?

Hey Joseph!If you are putting live plants in your aquarium you don't want to use the disposable ones at all. The carbon filtration takes dissolved organic carbons out of the water and plants need those.But... no plants, then no worries.I bought 4 of these. I put filter floss in two cartridges and bio media in another two cartridges. Bio media first to catch all the big stuff and get that great bacterial load growing which does most of your real waste management for you (biological filtration). That and your live plants ;)But even with no plants, and no need to avoid the carbon filter, the great thing about bio media is that to clean it you just give it a vigorous shake in a pail of old tank water (or clean de-chlorinated water)! That way you preserve your biological filter and never need to replace it. The bio media lasts indefinitely.Marineland PA0100 Bonded Filter Pad, 312-Square-Inchttp://www.amazon.com/gp/aw/d/B0002565PWSeachem Matrix Bio Media 1 Literhttp://www.amazon.com/gp/aw/d/B0002A5VIO

Would polyfill be ok in these? I have a 350 and plan on using the first two with polyfill and the back two as my biological/chemical filtration.

I think you can put whatever media you choose but you will have to cut the polyfill to fit each compartment because the cartridge is made for charcoal pellets, the inside has separate compartments. If you look closely at the photo, you will see horizontal lines separating vertical lines; each of those horizontal line is a divider.

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♻️ Elevate your aquarium’s clarity with refillable, mess-free filtration!

MAXIMIZED WATER FLOW - Ribbed cartridge structure promotes unrestricted water circulation for superior chemical and mechanical filtration.
MESS FREE MAINTENANCE - Snap-open design locks tight to prevent spills, making monthly refills hassle-free and clean.
SEAMLESS COMPATIBILITY - Perfectly engineered to fit Marineland Penguin 200 and 350 Power Filters—no guesswork, just plug & play.
TRUSTED AQUARIUM INNOVATION - Backed by 40+ years of Marineland expertise, ensuring your aquatic environment thrives with advanced filtration tech.
SUSTAINABLE FILTRATION POWER - Refillable cartridge lets you customize with carbon or specialty media for tailored water purity.

The Marineland Cartridge Media Refill is a refillable filtration cartridge designed for Penguin 200 and 350 Power Filters. Featuring a snap-open, spill-proof design and ribbed structure for optimal water flow, it allows monthly refills with carbon or other media to maintain clean, healthy aquarium water. Manufactured in the USA by Marineland, a leader in aquarium innovation for over 40 years.