MH-BSCS - Modified HEPES Brain Slice Cutting Solution (1000 ml)

Description

Modified HEPES Brain Slice (1000 ml) (MH-BSCS) is used for cutting, recovery and storage of acute brain slices from rats and mice. Product information Ready to Use MH-BSCS solution autoclaved Instructions for use: Ready to use: For 1000 ml MH-BSCS Solution: Oxygenate 500 ml Base A for 10 min Add slowly 500 ml Base B Oxygenate MH-BSCS Solution during use pH 7.4 when oxygenated with 95% O2, 5% CO2 (Carbogene) Use immediately once opened. Store at 4 - 8 °C Ional composition of MH-BSCS Solution NaCl = 92 mM NaHCO3 = 30 mM Glucose = 25 mM Na L-Ascorbate = 5 mM MgSO4 = 10 mM NaH2PO4 = 1,25 mM KCl = 2,5 mM CaCl2 = 0,5 mM HEPES = 20 mM Thiourea = 2 mM Na Pyruvate = 3 mM